FIGURE 9.

Influence of Glu on the Ca²⁺ dynamics in WT and preCGG cortical astrocytes. a and b, representative traces for Glu (10 μm) stimulation of Ca²⁺ response in WT (a) and preCGG (b) cortical astrocytes. Glu stimulated two distinct Ca²⁺ responses in both WT and preCGG cortical astrocytes. Some cells displayed more Ca²⁺ oscillations (solid trace), whereas the remaining responsive cells displayed sustained intracellular Ca²⁺ increase (dotted trace). Arrowheads indicate the addition of Glu (10 μm). c, quantification of the spontaneous Ca²⁺ oscillations before and after 10 μm Glu treatment. A concentration of 10 μm Glu increased the spontaneous Ca²⁺ oscillations in both WT and preCGG astrocytes. Each data point represents the mean ± S.E. from at least 150 cells for each group. d, quantification of the percentage of cells with sustained intracellular Ca²⁺ increase after treatment with 10 μm Glu in vehicle or 10 μm l-trans-PDC-exposed WT astrocytes and preCGG cortical astrocytes. Each data point represents the mean ± S.E. from at least 4 wells.