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. 2013 Mar 29;288(19):13863–13875. doi: 10.1074/jbc.M112.441444

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Restriction enzyme accessibility assay. A, representative polyacrylamide native gels of restriction enzyme accessibility near the DNA ends for intact (I), which contains a single uracil, or a single nucleotide gap (g) at the corresponding uracil location. Lanes M represent a radiolabeled 25-bp DNA ladder. HaeIII cleaves at +51. B, representative 16% polyacrylamide native gels of restriction enzyme accessibility near the dyad with HhaI, which cleaves at +2. C and D, plot of the mean ± 1 S.D. of three independent experiments.