TABLE 2.

Comparison of initial rates of cleavage of uracil by UDG and APE1 in NCPs

As in Table 1, the initial rates were calculated from the kinetic parameters obtained by fitting the data in Fig. 2A to a single-phase exponential equation (solid lines). Minor groove widths were estimated from the minor groove width plot reported by Ye et al. (36) for the DNA strand they designated as strand 2, which corresponds to our uracil-containing strand (supplemental Table S1).

Substrate	kNCP	Minor groove width
	% cleaved per min	Å
NCP-UI (+4)	4.48 ± 1.50	10
NCP-UI (+25)	2.37 ± 0.47	8
NCP-UI (−29)	5.11 ± 2.18	12
NCP-UI (−49)	10.78 ± 5.34	13
NCP-UO (+10)	40.36 ± 4.25	7
NCP-UO (+29)	24.56 ± 1.01	12
NCP-UO (−35)	23.64 ± 6.28	10