FIGURE 1.

Effect of KLF10 gene expression on myoblast proliferation. A, immunodetection of proliferating myoblasts and cells transfected with pCMVKLF10. Myoblasts were transfected with pCMVKLF10, and transfected cells were identified using a FLAG epitope tag antibody followed by Texas Red-conjugated secondary antibody. Proliferating cells were identified by immunodetection of PCNA using a fluorescein-conjugated anti-PCNA antibody. Nuclei were visualized by DAPI staining. Arrows indicate the nucleus of a transfected cell expressing the KLF10 gene but not PCNA. B, the percentage of PCNA-expressing cells with and without transfection of pCMVKLF10. Nontransfected and transfected cells were scored for expression of PCNA based on immunostaining with the PCNA antibody. Transfection of pCMVKLF10 significantly reduced the percentage of PCNA-positive cells (p < 0.01). Error bars indicate S.D.