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Equilibrium dissociation constant of ternary complex
The ternary complex was formed by titrating either factor or nucleotide in the absence or presence of EF-Ts.
| Dissociation constant KD |
||
|---|---|---|
| EF-Ts(−) | EF-Ts(+) | |
| nm | ||
| Factor | 47 ± 3.1 | 12.6 ± 1.1 |
| GTP | 195 ± 25 | 685 ± 35 |
| GDPNP | 7000 ± 100 | 9270 ± 105 |
| GDPγS | 240 ± 18 | 490 ± 41 |
