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. Author manuscript; available in PMC: 2013 May 10.
Published in final edited form as: Sci Signal. 2009 Sep 22;2(89):ra57. doi: 10.1126/scisignal.2000205

Fig. 3.

Fig. 3

Knock-down of MSK2 results in increased apoptosis and Noxa induction. (A) U2OS cells were transfected with control, Mdm2, or MSK2 siRNAs for 48 hours and treated with the indicated p53-inducing agents for an additional period of 24 hours. Apoptosis was analyzed by flow cytometry. Error bars represent SDM from at least three samples. Statistical analysis was determined by t-test. * P < 0.05; *** P < 0.001. (B) U2OS, HCT116 and SJSA1 cells were either irradiated (UVC) or treated with cisplatin (CIS) or neocarzinostatin (NCS) for the indicated times. Protein abundance was analyzed by Western blotting. (C) U2OS cells were transfected with control or p53 siRNAs and 48 hours later, were treated with UVC or CIS for 24 additional hours. Protein abundance was analyzed by Western blotting.