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. 2013 Mar 20;5:plt021. doi: 10.1093/aobpla/plt021

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Published by Oxford University Press on behalf of the Annals of Botany Company.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Differential methylation of the ACS1 promoter. The relative methylation state of six different regions of the ACS1 promoter were analysed from genomic DNA isolated from five different orchards at harvest. Genomic DNA was extracted from the peel of fruit and digested with an endonuclease that specifically digests methylated DNA. This DNA was then subjected to qPCR for each primer pair on the ACS1 promoter, in both undigested and digested samples. The cycle threshold values of undigested DNA were normalized for digested DNA and presented as relative methylation.