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. 2013 Mar 20;5:plt021. doi: 10.1093/aobpla/plt021

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Published by Oxford University Press on behalf of the Annals of Botany Company.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Relationship between DNA methylation of the ACS1 promoter and IEC and external CO₂ injury occurrence. (A) ACS1pro1 region, IEC. (B) ACS1pro1 region and injury. (C) ACS3pro1 region, IEC. (D) ACS1pro3 region and injury. Genomic DNA was extracted from the peel of fruit and digested with an endonuclease that specifically digests methylated DNA. This DNA was then subjected to qPCR for each primer pair on the ACS1 promoter, in both undigested and digested samples. The cycle threshold values of undigested DNA were normalized for digested DNA and presented as relative methylation.