Fig 2.

Putative operon organization of AM-19226 T3SS genomic island. Heat map representation of the AM-19226 T3SS genomic island spanning from A33_1660a (AcfA) to A33_1706 (CHP) as viewed in the IGV. The lines above the map indicate three major operons. The operons encode genes for structural proteins (vcsRTCNS2, vcsVUQ2, vcsJ2, and vspD) and some effector proteins (vopA, vopH, vopI, vopM, and vopW). Additional effector genes in the 5′ and 3′ flanking regions, as well as acfD, encoding a putative accessory colonization factor, are also indicated. All genes are shown aligned to the IGV heat map with the following settings: positive strand, base count 0 to 100 in gray, 100 to maximum in black; negative strand, 0 to 50 in gray, 50 to maximum in black.