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VttRB and ToxR negatively impact biofilm formation. Static biofilms were measured after incubation at 30°C for 24 h. Total biofilm biomass was determined by measuring absorbance at 570 nm. *, P < 0.0016, and **, P < 0.0001 compared to the wild-type strain. A representative of three independent experiments with n = 6 for each strain is shown.
