Table 2.

Relative CyaA′ expression from infected J774 cells^a

Effector locus	Δhfq mutant vs WT
	cyaA′ transcript level		CyaA′ protein level
	UTR fusion	Intact effector	UTR fusion	Intact effector
gtgA	1.4 ± 1.8	1.4 ± 1.2	0.8 ± 0.3	0.2 ± 0.1*
cigR	1.7 ± 1.1	1.2 ± 1.0	4.5 ± 6.3	0.6 ± 0.1
gogB	2.1 ± 0.6*	0.9 ± 0.4	1.5 ± 0.2	0.7 ± 0.2
sseL	1.9 ± 0.9	0.5 ± 0.3	3.1 ± 3.6	0.5 ± 0.1
steD	1.7 ± 1.1	0.4 ± 0.3*	0.9 ± 0.1	0.5 ± 0.1

^aFor cyaA′ transcript levels, RNA was collected from infected J774 macrophages and analyzed by RT-PCR to determine cyaA′ RNA levels in the hfq mutant relative to WT bacteria. For CyaA′ protein levels, lysates from infected J774 cells were evaluated by Western blotting for CyaA′ and DnaK. Representative blots are shown in Fig. S4 in the supplemental material. Protein levels in the hfq mutant are relative to levels in WT bacteria. Standard deviation denotes the error of results from at least three independent experiments. Both ANOVA and the Student t test were used to calculate significant differences.

^*indicates a P of ≤0.05.