Fig 3.

Effects of PelA catalytic mutations on biofilm formation. (A) Colony morphology (top) and pellicle formation (bottom) are shown. (B) Microtiter dish biofilm assay of P. aeruginosa PA14, PA14ΔpelA, and the indicated pelA point mutants are shown. (C) Western blots for PelA (top), PelC (middle) and RNA polymerase (RNAp), a protein loading control (bottom) are shown for PA14, PA14ΔpelA, and the indicated pelA point mutants expressing the diguanylate cyclase PA1120 (pJN1120). The asterisk indicates the PelA protein. (D) Microtiter dish biofilm assay for strains expressing pJN1120 is shown. Error bars represent standard deviations.