Fig 5.
PelA localizes primarily to the periplasmic and membrane fractions. Western blots of whole-cell (WC), cytoplasmic (C), periplasmic (P), and membrane (M) fractions are shown for PAO1ΔwspFΔpsl PBADpel expressing pPSV18, a plasmid that synthesizes β-lactamase. Subcellular fractions were probed for PelA, PelC (membrane protein), RNA polymerase (RNAp; cytosolic protein), and β-lactamase (Bla; periplasmic protein). The asterisk indicates that the band for PelA protein is the lower of the two immunoreactive bands.