Fig 6.

Effects on the motility from S. acidocaldarius by interfering with the N-glycan assembly. Equivalent amounts of cells (5 to 6 μl) from S. acidocaldarius ΔaapF ΔflaH (lane 1), S. acidocaldarius ΔaapF (lane 2), the background strain S. acidocaldarius MW001(lane 3), S. acidocaldarius Δagl3 (lane 4), S. acidocaldarius Δagl16, and S. acidocaldarius Δagl16 complemented with pSVA1274 (lane 6), were dripped onto a 0.15% Gelrite plate and incubated for 4 and 9 days at 75°C. The panels on the right show the enlarged colony border and the motility zone (taken from the left panel: 0 mM NaCl, 9-day incubation), as well as the N-glycan composition of MW001 and the Δagl16 and Δagl3 mutants. The ability to swim corresponds to the N-glycan size.