Table 1.
Plasmids used in this study
| Plasmid | Insert | Reference |
|---|---|---|
| pSAV407 | Gene targeting plasmid, pGEM-T Easy backbone, pyrEF cassette of S. solfataricusa | 28 |
| pSAV1233 | In-frame deletion of agl16 cloned into pSVA407 with ApaI and BamHI | This study |
| pMZ1 | Gene targeting plasmid with a C-terminal Strep tag and 10× His tag | 30 |
| pSAV1450 | pRN1-based shuttle vector with lacS reporter gene of S. solfataricusb | 28 |
| pSVA1273 | Full agl16 gene insert cloned into pMZ1 with NcoI and BamHI | This study |
| pSAV1274 | agl16 insert from pSVA1273 cloned into pSVA1450 with NcoI and EagI | This study |
a
pyrEF encode the enzymes orotate phosphoribosyltransferase and orotidine-5-mono-phosphate decarboxylase, respectively.
b
lacS encodes β-d-galactosidase of S. solfataricus.