Table 2.

Oligonucleotide primers used in this study

Primer	Sequence (5′–3′)a	Restriction site	Description and target gene
1848	CTCTACGGGCCCACTTCTGTCTCCCCACTGG	ApaI	Up-for, Δagl16
1849	AAGGTCTGGATGTTGTAAAAATTATGTCCATGACATCACAGGACA		Up-rev, Δagl16
1850	TGGACATAATTTTTACAACATCCAGACCTTATACATGAATCATCC		Down-for, Δagl16
1851	CGCCGAGGATCCAACCATGGAATTGTAGCC	BamHI	Down-rev, Δagl16
4101	GTCCTCAAGACCCAATTCACTAAC		RT-PCR-for, agl16
4106	GCCGACTGCAGCATTAGTAATGACTCCACACCAC		RT-PCR-rev, agl16
2799	CATGCGGATCCTTAACTTAAACTTTTATAGAGCTCAAGC	BamHI	Forward primer complementing agl16
4100	CATGCCCATGGATGTATAAGGTCTGGATGTTGACCCCAC	NcoI	Reverse primer complementing agl16
1729	CTGCTGCAATTACAGCGTTC		RT-PCR-for aglB
1730	AACCGTGAGCTACTTCAGAC		RT-PCR-rev aglB

^aUnderlining indicates restriction sites.