Table 2.
Primer | Sequence (5′–3′)a | Restriction site | Description and target gene |
---|---|---|---|
1848 | CTCTACGGGCCCACTTCTGTCTCCCCACTGG | ApaI | Up-for, Δagl16 |
1849 | AAGGTCTGGATGTTGTAAAAATTATGTCCATGACATCACAGGACA | Up-rev, Δagl16 | |
1850 | TGGACATAATTTTTACAACATCCAGACCTTATACATGAATCATCC | Down-for, Δagl16 | |
1851 | CGCCGAGGATCCAACCATGGAATTGTAGCC | BamHI | Down-rev, Δagl16 |
4101 | GTCCTCAAGACCCAATTCACTAAC | RT-PCR-for, agl16 | |
4106 | GCCGACTGCAGCATTAGTAATGACTCCACACCAC | RT-PCR-rev, agl16 | |
2799 | CATGCGGATCCTTAACTTAAACTTTTATAGAGCTCAAGC | BamHI | Forward primer complementing agl16 |
4100 | CATGCCCATGGATGTATAAGGTCTGGATGTTGACCCCAC | NcoI | Reverse primer complementing agl16 |
1729 | CTGCTGCAATTACAGCGTTC | RT-PCR-for aglB | |
1730 | AACCGTGAGCTACTTCAGAC | RT-PCR-rev aglB |
Underlining indicates restriction sites.