Strains |
|
|
E. coli
|
|
|
DH5α |
Cloning strain |
Invitrogen |
Y. pseudotuberculosis
|
|
|
IP2666/pIB1 |
Wild type |
32 |
IP2666 ΔyopB
|
Deletion of yopB (codons 19–346) |
33 |
IP2666 ΔyscF
|
Deletion of yscF (codons 2–86) |
29 |
IP2666 ΔlcrV
|
Deletion of lcrV (codons 19–326) |
This study |
IP2666 ΔyopN
|
Deletion of yopN (codons 2–287) |
29 |
IP2666 Δ5 |
Deletion of yopHEMOJ
|
34 |
IP2666 Δ5V |
Deletion of yopHEMOJ and lcrV
|
This study |
IP2666 Δ5F |
Deletion of yopHEMOJ and yscF
|
This study |
IP2666 Δ5N |
Deletion of yopHEMOJ and yopN
|
This study |
IP2666 Δ5NV |
Deletion of yopHEMOJN and lcrV
|
This study |
IP2666 Δ5NF |
Deletion of yopHEMOJN and yscF
|
This study |
Plasmids |
|
|
pCVD442 |
Suicide vector; Apr
|
35 |
pCVD442-lcrVKO |
Suicide vector for deleting lcrV; Apr
|
This study |
pCVD442-yscFKO |
Suicide vector for deleting yscF; Apr
|
29 |
pCVD442-yopNKO |
Suicide vector for deleting yopN; Apr
|
29 |
pTRC99A |
IPTG-inducible vector, Apr
|
36 |
pTRC99A-yscF
|
Expresses yscF
|
29 |
pTRC99A-yscFD28AD46A
|
Expresses yscFD28AD46A
|
This study |