Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | Cloning strain | Invitrogen |
| Y. pseudotuberculosis | ||
| IP2666/pIB1 | Wild type | 32 |
| IP2666 ΔyopB | Deletion of yopB (codons 19–346) | 33 |
| IP2666 ΔyscF | Deletion of yscF (codons 2–86) | 29 |
| IP2666 ΔlcrV | Deletion of lcrV (codons 19–326) | This study |
| IP2666 ΔyopN | Deletion of yopN (codons 2–287) | 29 |
| IP2666 Δ5 | Deletion of yopHEMOJ | 34 |
| IP2666 Δ5V | Deletion of yopHEMOJ and lcrV | This study |
| IP2666 Δ5F | Deletion of yopHEMOJ and yscF | This study |
| IP2666 Δ5N | Deletion of yopHEMOJ and yopN | This study |
| IP2666 Δ5NV | Deletion of yopHEMOJN and lcrV | This study |
| IP2666 Δ5NF | Deletion of yopHEMOJN and yscF | This study |
| Plasmids | ||
| pCVD442 | Suicide vector; Apr | 35 |
| pCVD442-lcrVKO | Suicide vector for deleting lcrV; Apr | This study |
| pCVD442-yscFKO | Suicide vector for deleting yscF; Apr | 29 |
| pCVD442-yopNKO | Suicide vector for deleting yopN; Apr | 29 |
| pTRC99A | IPTG-inducible vector, Apr | 36 |
| pTRC99A-yscF | Expresses yscF | 29 |
| pTRC99A-yscFD28AD46A | Expresses yscFD28AD46A | This study |