Table 1.
Bacterial strains, plasmids, and oligonucleotides used in this study
| Strain, plasmid, or oligonucleotide | Genotype, phenotype, and/or sequence (5′–3′)a | Source or reference |
|---|---|---|
| Strains | ||
| D. dadantii | ||
| 3937 | Wild type | Laboratory collection |
| A1524 | rafR ganB pecS::Mu-Cmr | 45 |
| A3953 | pecS::Mu-Cmr | 45 |
| A4239 | gacA::Cmr | 40 |
| A4114 | kdgR::Smr | Laboratory collection |
| A4415 | pelD::uidA-Kmr | 36 |
| A5051 | pelN::uidA-Kmr | This study |
| A5185 | rafR ganB pecS::Mu-Cmr pelN::uidA-Kmr | This study |
| A5186 | rafR ganB pecS::Mu-Cmr pelL::uidA-Kmr | This study |
| A5189 | rafR ganB ΔoutD pecS::Mu-Cmr pelN::uidA-Kmr | This study |
| A5190 | rafR ganB ΔoutD pecS::Mu-Cmr pelL::uidA-Kmr | This study |
| A5194 | gacA::Cmr pelN::uidA-Kmr | This study |
| A5218 | pecS::Mu-Cmr pelN::uidA-Kmr | This study |
| A5293 | gacA::Cmr pelL::uidA-Kmr | This study |
| A5298 | pelL::uidA-Kmr | 10 |
| A5299 | kdgR::Smr pelN::uidA-Kmr | This study |
| E. coli | ||
| NM522 | Δ(lac-proAB) Δ(mcrB-hsdSM)5 supE thi [F′ proAB lacIq30 lacZΔM15] | Laboratory collection |
| BL21(DE3) | E. coli B, F− dcm ompT hsdS gal λ(DE3), T7 polymerase gene under the lacUV5 promoter | 29 |
| Plasmids | ||
| pGEM-T | lacZ; Apr | Promega |
| pBS Ap | pBluescript SK(+); Apr | Stratagene |
| pT7-5 | T7 phi10; Apr | 28 |
| pI2805 | pBS Ap; pelN+ | This study |
| pI2812 | pBS Ap; pelN::uidA-Kmr | This study |
| pI3372 | pGEM-T; pelN+ | This study |
| pNA13 | pT7-5 derivative with a 1.4-kb BamHI-XbaI fragment from pI3372, pelN+ | This study |
| Oligonucleotides | ||
| PelNG | GCGGATCCCGGTAAATGCTTTGGGTTTG | This study |
| PelND | CGTCTAGAGTTTCGCCGCTTATTTCAGTTC | This study |
a
Cmr, chloramphenicol resistance; Apr, ampicillin resistance; Smr, streptomycin resistance; Kmr, kanamycin resistance.