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Effect of betaine catabolism on the composition of the accumulated compatible solutes of B728a (A and C) and the ΔgbcAB mutant (B and D) after 5 h (A and B) and 30 h (C and D) of growth. Cells were collected for 13C-NMR at 5 and 30 h after cultures were amended with 0.6 M NaCl and betaine. Resonances (peaks) corresponding to betaine (B), l-glutamate (G), the dipeptide NAGGN (N), and trehalose (T) are indicated. Asterisks indicate peaks for residual ethanol from sample preparation.
