Fig 5.

Role of betaine catabolism in relieving betaine-mediated inhibition of endogenous solute synthesis. (A and B) qRT-PCR analysis of ggnA (A) and treX (B) in B728a and the ΔgbcAB mutant at 5 and 30 h after amending cells with 0.6 M NaCl and betaine. Values are expressed relative to transcript levels at 0 M NaCl without betaine. Values indicated with the same letter do not differ significantly (P < 0.05). (C) B728a and the Δggn Δtre and ΔgbcAB mutants were grown in MinA-succinate with 0.6 M NaCl and betaine, and growth in microtiter plates was monitored based on optical density. Values are means ± SEM (n = 3 for panels A and B; n = 4 for panel C).