Fig 5.

Isolation and identification of methenyl-dH₄MPT in the mtdB mutant during growth on methylamine. (A) Identification of the maximal absorbance points of the purified compounds and MS analysis of reaction products (insets: left, dH₄MPT; right, methenyl-dH₄MPT). Both species were compared to standards. amu, atomic mass units. (B) Accumulation of dH₄MPT and methenyl-dH₄MPT in the mtdB mutant strain (gray bars) compared to the wild-type strain (black bars). (C) Growth of wild-type M. extorquens (crosses) and mtdB mutant strain with empty vector (open triangles) and overexpressing mch (filled triangles). All strains were pregrown on succinate and inoculated in medium containing methylamine (35 mM).