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Specific activity of the methylene-H4MPT dehydrogenase MtdA in cell extracts with methylene-H4F as the substrate and NADP+ as the cosubstrate
| Strain | Sp act (mU/mg) with 20 μM H4F |
Fold change | |
|---|---|---|---|
| Normal extracts | Desalted extracts | ||
| WT | 0.23 ± 0.09 | 0.36 ± 0.09 | 1.5 |
| mtdB mutant | 0.34 ± 0.1 | 1.23 ± 0.1 | 3.6 |
| mtdB mutant/pNM3 | 0.13 ± 0.12 | 1.38 ± 0.15 | 10 |
