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Kinetic parameters of the methylene-H4MPT dehydrogenase MtdA with NADPH as a cosubstrate in the presence and absence of methenyl-dH4MPTa
| Substrate | Km (μM) | Vmax (U/mg) |
|---|---|---|
| Methenyl-H4F | 45 | 103 |
| Methenyl-H4F + methenyl-dH4MPT | 187 | 135 |
Concentrations of methenyl-H4F varied from 10 μM to 100 μM; the concentration of methenyl-dH4MPT was 100 μM.
