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Isoflurane activates intestinal sphingosine kinase (SK) 1, but not SK2, messenger RNA (mRNA) expression. (A) Representative gel images of RT-PCR (of 4 experiments) of SK1, SK2, and GAPDH from the small intestines of sham mice exposed to 4 h of pentobarbital or 1.2% isoflurane. (B) Densitometric quantifications of band intensities relative to GAPDH from RTPCR reactions. * P less than 0.05 versus. Pentobarbital sham group. Data presented as mean ± SEM.
