Table 5.

Myosin protein post-translational modifications in HRT using (U) and non-using (NU) monozygotic twin pairs

Modification type	Peptide sequence	Amino acid modified	HRT user or non-user	Myosin isoforms	Amino acid position	Domain
Carbonylation	KMEGDLNEMEIQLNHANR	D/K	5U 1NU	I/IIa	1623/1627	Tail domain
Carbonylation	LQTESGEFSR	F	5U 2NU	IIa	1293	Tail domain
Carbonylation	SAETEKEMATMKEEFQK	K	4U 2NU	IIa	853	Tail domain
Carbonylation	VKVGNEFVTK	F	3U 6NU	I/IIa	413	S1 myosin head (CM-loop)
Deamidation	AEDEEEINAELTAK	N	1U 5NU	IIa	940	Tail domain
Deamidation	LAQESIMDIENEK	N	2U 4NU	IIa	1079	Tail domain*
Methylation	QAEEAEEQANTNLSK	E	4U 1NU	IIa	1893	Tail domain
Methylation	TNAACAALDKK	D	2U 4NU	IIa	1449	Tail domain

The modified MyHC isoform, amino acid, amino acid position and the domain of the modified myosin are presented.

^*This deamidation was observed in older but not in younger controls.