Liposomes prepared with or without cholesterol were loaded with prenylated Rab9 as described under “Experimental Procedures.” A, top, dot-blot analysis of liposome-associated Rab9 following treatment with the indicated amounts of GDI and subsequent isolation on a Nycodenz gradient.A,bottom, quantitation of dot-blots from three independent experiments, shown is the mean percentage of liposomal-associated Rab9 following GDI treatment compared with non-GDI treated samples. Error bars represent S.D. B, top, same as A, top, but for Rab5. B, bottom, quantitation of dot-blots shown at the top of panel B.