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. Author manuscript; available in PMC: 2013 May 10.
Published in final edited form as: J Biol Chem. 2006 Apr 26;281(26):17890–17899. doi: 10.1074/jbc.M601679200

Figure 7. GDI extraction of Rabs from liposomes is impaired in the presence of cholesterol.

Figure 7

Liposomes prepared with or without cholesterol were loaded with prenylated Rab9 as described under “Experimental Procedures.” A, top, dot-blot analysis of liposome-associated Rab9 following treatment with the indicated amounts of GDI and subsequent isolation on a Nycodenz gradient.A,bottom, quantitation of dot-blots from three independent experiments, shown is the mean percentage of liposomal-associated Rab9 following GDI treatment compared with non-GDI treated samples. Error bars represent S.D. B, top, same as A, top, but for Rab5. B, bottom, quantitation of dot-blots shown at the top of panel B.