Table 2.
Bacterial strains used in this study.
| Strain | Description | Source |
|---|---|---|
| E. coli XL1 blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZΔM15 Tn10 (Tcr)] | Stratagene |
| E. coli XL2 blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZΔM15 Tn10 (Tcr) Amy Cmr] | Stratagene |
| E. coli S17-1 | Used for mating constructs into P. aeruginosa; thi pro hsdR recA RP4-2 (Tc::Mu) (Km::Tn7) | Simon et al. (1983) |
| PA103 | Virulent lung isolate of P. aeruginosa, known type III-secreted effector proteins are ExoT and ExoU | Liu (1966) |
| PA103pscJ::Tn5 | Non-cytotoxic and defective in type III secretion; Tcr | Kang et al. (1997) |
| ΔrtsM | PA103 strain containing an in-frame deletion of amino acids 37–924 of rtsM ORF | This study |
| ΔexsA | PA103 strain containing an in-frame deletion of exsA; defective in expression of type III secretion genes | Schulert et al. (2003) |
| PA103 attB::Gm | Gmr cassette integrated at the attB site of the PA103 chromosome; Gmr | This study |
| PA103 pARA | PA103 carrying empty pARA vector; Cbr | This study |
| ΔrtsM attB::Gm | Gmr cassette integrated at the attB site of the ΔrtsM chromosome; Gmr | This study |
| ΔrtsM attB::rtsM | RtsM under control of its own promoter integrated at the attB site of the ΔrtsM chromosome | This study |
| ΔrtsM pMMB67EH | ΔrtsM carrying empty pMMB67EH vector; Cbr | This study |
| ΔrtsM pMMB-RtsM | ΔrtsM carrying pMMB-RtsM expressing RtsM from IPTG inducible tac promoter; Cbr | This study |
| ΔrtsM pRtsM-BB2 | ΔrtsM carrying pRtsM-BB2 expressing RtsM with a C-terminal BB2 tag under the control of its own promoter; Cbr | This study |
| ΔrtsM pARA | ΔrtsM carrying empty pARA vector; Cbr | This study |
| ΔrtsM pARA-Vfr | ΔrtsM carrying pARA-Vfr expressing Vfr from an arabinose inducible promoter; Cbr | This study |
| ΔrtsM pARA-ExsA | ΔrtsM carring pARA-ExsA expressing ExsA from an arabinose inducible promoter; Cbr | This study |
| PA103 attB::lux | Promoterless lux fusion integrated at the attB site of the PA103 chromosome | This study |
| ΔrtsM attB::lux | Promoterless lux fusion integrated at the attB site of the ΔrtsM chromosome | This study |
| ΔexsA attB::lux | Promoterless lux fusion integrated at the attB site of the ΔexsA chromosome | This study |
| PA103 exoS::lux | lux fusion with exoS promoter integrated at the attB site of the PA103 chromosome | This study |
| ΔrtsM exoS::lux | lux fusion with exoS promoter integrated at the attB site of the ΔrtsM chromosome | This study |
| ΔexsA exoS::lux | lux fusion with exoS promoter integrated at the attB site of the ΔexsA chromosome | This study |
| PA103 exoT::lux | lux fusion with exoT promoter integrated at the attB site of the PA103 chromosome | This study |
| ΔrtsM exoT::lux | lux fusion with exoT promoter integrated at the attB site of the ΔrtsM chromosome | This study |
| ΔexsA exoT::lux | lux fusion with exoT promoter integrated at the attB site of the ΔexsA chromosome | This study |
| PA103 exoU::lux | lux fusion with exoU promoter integrated at the attB site of the PA103 chromosome | This study |
| ΔrtsM exoU::lux | lux fusion with exoU promoter integrated at the attB site of the ΔrtsM chromosome | This study |
| ΔexsA exoU::lux | lux fusion with exoU promoter integrated at the attB site of the ΔexsA chromosome | This study |
| PA103 pscN::lux | lux fusion with pscN operon promoter integrated at the attB site of the PA103 chromosome | This study |
| ΔrtsM pscN::lux | lux fusion with pscN operon promoter integrated at the attB site of the ΔrtsM chromosome | This study |
| ΔexsA pscN::lux | lux fusion with pscN operon promoter integrated at the attB site of the ΔexsA chromosome | This study |
| PA103 popN::lux | lux fusion with popN operon promoter integrated at the attB site of the PA103 chromosome | This study |
| ΔrtsM popN::lux | lux fusion with popN operon promoter integrated at the attB site of the ΔrtsM chromosome | This study |
| ΔexsA popN::lux | lux fusion with popN operon promoter integrated at the attB site of the ΔexsA chromosome | This study |
| PA103 pcrG::lux | lux fusion with pcrG operon promoter integrated at the attB site of the PA103 chromosome | This study |
| ΔrtsM pcrG::lux | lux fusion with pcrG operon promoter integrated at the attB site of the ΔrtsM chromosome | This study |
| ΔexsA pcrG::lux | lux fusion with pcrG operon promoter integrated at the attB site of the ΔexsA chromosome | This study |
| PA103 exsD::lux | lux fusion with exsD operon promoter integrated at the attB site of the PA103 chromosome | This study |
| ΔrtsM exsD::lux | lux fusion with exsD operon promoter integrated at the attB site of the ΔrtsM chromosome | This study |
| ΔexsA exsD::lux | lux fusion with exsD operon promoter integrated at the attB site of the ΔexsA chromosome | This study |
| PA103 exsC::lux | lux fusion with exsC operon promoter integrated at the attB site of the PA103 chromosome | This study |
| ΔrtsM exsC::lux | lux fusion with exsC operon promoter integrated at the attB site of the ΔrtsM chromosome | This study |
| ΔexsA exsC::lux | lux fusion with exsC operon promoter integrated at the attB site of the ΔexsA chromosome | This study |
| PA103 cyaB::lux | lux fusion with cyaB promoter integrated at the attB site of the PA103 chromosome | This study |
| ΔrtsM cyaB::lux | lux fusion with cyaB promoter integrated at the attB site of the ΔrtsM chromosome | This study |
| ΔexsA cyaB::lux | lux fusion with cyaB promoter integrated at the attB site of the ΔexsA chromosome | This study |
| PA103 vfr::lux | lux fusion with vfr promoter integrated at the attB site of the PA103 chromosome | This study |
| ΔrtsM vfr::lux | lux fusion with vfr promoter integrated at the attB site of the ΔrtsM chromosome | This study |
| ΔexsA vfr::lux | lux fusion with vfr promoter integrated at the attB site of the ΔexsA chromosome | This study |
| PA103 zwf::lux | lux fusion with zwf promoter integrated at the attB site of the PA103 chromosome | This study |
| ΔrtsM zwf::lux | lux fusion with zwf promoter integrated at the attB site of the ΔrtsM chromosome | This study |
| ΔexsA zwf::lux | lux fusion with zwf promoter integrated at the attB site of the ΔexsA chromosome | This study |