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. 2012 Nov 27;54(3):401–408. doi: 10.1093/jrr/rrs113

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© The Author 2012. Published by Oxford University Press on behalf of The Japan Radiation Research Society and Japanese Society for Therapeutic Radiology and Oncology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 6. — (A) The number of γH2AX foci per nucleus in miRNA-22 knockdown EC9706 cells was significantly lower than that of control cells after 1 h post-IR (P < 0.01). Data bars shown represent the mean ± SD of γH2AX foci in unirradiated and irradiated cells. Two hundred nuclei were analyzed per condition. (B) The percentage of apoptotic EC9706 cells in the miRNA-22 knockdown group was significantly lower than in control groups after radiation (P < 0.05). (C) The number of γH2AX foci per nucleus in KYSE150 cells with miRNA-22 higher expression was significantly higher than in control cells (P < 0.05). (D) The percentage of apoptotic cells in the forced miRNA-22 expression group was significantly higher than in control groups (P < 0.05).