Plot of [θ]200 verse [θ]222 shows that the Bacillus subtilis, Escherichia coli and Salmonella typhimurium FlgM proteins are in a premolten globule state. The [θ]200 verse [θ]222 positions for the B. subtilis (BS), E. coli (EC), Proteus mirabilis (PA), Pseudomonas aeruginosa (PA), and S. typhimurium (ST) FlgM proteins were plotted together. The circle in the upper left of the figure represents the coil-like region and the circle in the center of the figure shows the region for the premolten globular state, as determined by Uversky [53].