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. Author manuscript; available in PMC: 2013 May 10.
Published in final edited form as: J Immunother. 2012 May;35(4):329–336. doi: 10.1097/CJI.0b013e318255adcc

Figure 2. Expression profile of chemokine receptors by differentially cultured Tregs.

Figure 2

(A) Analysis of chemokine receptors in freshly isolated nTregs and ex vivo cultured eTregs and rTregs. Shown are flow cytometry dot plots for nTregs, eTregs and rTregs, stained with anti-CXCR4, CCR4, CCR5, CXCR3, CCR1, CCR3, CCR9 and CCR7 fluorochrome-conjugated mAb from 1 representative donor. The percentages shown represent the double positive cells (chemokine receptor and CD25+). (B) Comparative phenotypic analysis of chemokine receptors expression in freshly isolated nTregs and ex vivo cultured eTregs and rTregs. Bars (white for nTregs; black for eTregs and gray for rTregs) represent mean ± SD of 5 different donors.