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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: J Immunol. 2012 Jul 30;189(5):2478–2487. doi: 10.4049/jimmunol.1103786

Figure 10. H-ficolin/MASP-2 complexes fix complement in presence of IAV.

Figure 10

ELISA plates were coated with IAV as in figure 1. H-ficolin/MASP-2 complexes were purified from human serum and incubated with the surface bound IAV as described in “Materials and Methods”. Complement fixation was detected using antibody to complement component C4. H-ficolin caused dose related deposition of C4 onto the viral surface (n=5; * indicates p<0.05 vs. IAV-coated plates incubated with complement in the absence of H-ficolin. As a positive control 2μg/ml of the H-ficolin/MASP-2 complex was incubated with wells coated with acetylated BSA. This resulted in fixation of complement as reported (mean±SEM OD450 for C4 was 1.2±0.1).