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. Author manuscript; available in PMC: 2013 May 10.
Published in final edited form as: Mol Biochem Parasitol. 2007 Feb 21;153(2):95–106. doi: 10.1016/j.molbiopara.2007.02.003

Table 4. Trans-spliced and non-trans-spliced mRNAs have similar translation efficiencies and functional half-lives following introduction into Ascaris embryos.

The illustrated RNAs were introduced into Ascaris embryos and samples of embryos collected at time intervals and assayed for luciferase activity. The time course of luciferase activity was used to calculate translation efficiency (Relative luciferase light units/sec/min), functional half-life (min), and maximum accumulation of luciferase (Relative luciferase light units/sec) as described in Materials and Methods. Data illustrate a representative experiment and represent the mean of triplicate transfections.

RNA Translational Efficiency (RLU/sec/min) Relative Rate of Translation Functional half-life (min) Relative mRNA Stability Maximum Accumulation (RLU/sec) Relative Level of Expression
m7GpppG-Ren-A85 979 1.0 56 1.0 158,479 1.0
m7GpppG-SL-Ren-A85 820 0.8 43 0.8 62,420 0.4
m2,2,7GpppG-Ren-A85 396 0.4 57 1.0 49,036 0.3
m2,2,7GpppG-SL-Ren-A85 850 0.9 54 1.0 137,175 0.9