Hypothalamic sections from Sham ADX (A, E), ADX (B, F), and ADX + Dex (C, G) treated rats were examined for CRH-ir (red) and pan-Ac H3- or 4-ir (green). (D,H) The number of CRH-ir cells that display either Ac-H3- or Ac-H4- ir increases after ADX. This increase is attenuated in the presence of Dex. Bars: Low magnification =100µm; high magnification (inset) = 20µm. (*) Significantly different from SHAM and ADX+DEX p<0.05. (▲) co-localization.