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. Author manuscript; available in PMC: 2013 May 10.
Published in final edited form as: Sci Transl Med. 2012 May 9;4(133):133ra57. doi: 10.1126/scitranslmed.3003425

Figure 4. Longitudinal clone tracking and analysis of RIS-associated gene transcript expression.

Figure 4

Panels A and B: Agarose gel electrophoresis of PCR products obtained using primer sets specific for either PRDM16 Clone 3087539 or PRDM16 Clone 2988812 (A), HMGA2 Clone 3087539 (B), the MND provirus backbone as a control for presence of gene modified cells or β-Actin as a control for DNA template integrity in the PCR reaction. Bands observed at a different molecular weight in the topmost panel were determined to be an artifact of the PCR reaction by sequencing analysis. Panels C and D: Agarose gel electrophoresis of PCR products obtained using primer sets for PRDM16 transcript spanning the exon 16:exon 17 junction (C), HMGA2 transcript spanning the exon 4:exon 5 junction (D), MGMT transcripts or GAPDH transcripts (housekeeping gene control).