TABLE 1.
Breakdown of genome-wide QTL fine-mapped in this study
| Chr | QTL | Trait* | LOD | POS (Mb) | Proximal CI | Distal CI | Interaction¶
|
Total Genes | Glucose and/or Insulin Affecting Genes | ||
|---|---|---|---|---|---|---|---|---|---|---|---|
| ADD | DOM | IMP | |||||||||
| 1 | Ddiab1a | AUC_20wks | 3.32 | 67.99 | 66.31 | 68.56 | sd | s | 0 | 9 | Acadl |
| Ddiab1b | bGLC_20wks | 3.79 | 77.73 | 76.25 | 78.28 | d | sd | 0 | 2 | ||
| Ddiab1c | AUC_10wks | 3.35 | 190.62 | 189.77 | 191.29 | 0 | sd | 0 | 4 | ||
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| 2 | Ddiab2a | AUC_10wks | 4.06 | 59.26 | 58.53 | 61.58 | sd | s | 1 | 15 | |
| Ddiab2b | INS | 4.13 | 69.20 | 67.96 | 70.35 | sd | 0 | d | 23 | G6pc2 | |
| Ddiab2c | GLC | 3.30 | 73.81 | 71.73 | 76.29 | 1 | 0 | 0 | 39 | Rapgef4 | |
| Ddiab2d | GLC | 3.38 | 99.06 | 93.29 | 99.82 | 1 | 0 | s | 14 | ||
| Ddiab2e | INS | 3.60 | 103.71 | 102.74 | 106.63 | 0 | d | 0 | 34 | ||
| Ddiab2f | AUC_20wks | 4.18 | 145.71 | 145.01 | 147.25 | 0 | 0 | s | 13 | Insm1, Nkx2-2 | |
|
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| 3 | Ddiab3a | AUC_20wks | 3.55 | 15.21 | 14.27 | 16.50 | 0 | s | d | 12 | |
| Ddiab3b | AUC_10wks | 5.61 | 61.07 | 59.44 | 66.07 | 1 | 0 | 0 | 32 | Sucnr1 | |
| Ddiab3c | INS | 3.57 | 135.46 | 133.52 | 135.82 | 0 | sd | sd | 27 | Fabp2 | |
|
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| 4 | Ddiab4a | AUC_20wks | 3.58 | 55.34 | 55.34 | 60.38 | 0 | 0 | sd | 40 | |
| Ddiab4b | AUC_20wks | 3.68 | 122.63 | 120.73 | 123.57 | sd | 0 | 0 | 26 | ||
| Ddiab4c | AUC_20wks | 3.80 | 151.63 | 150.49 | 152.94 | 0 | 0 | sd | 23 | ||
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| 5 | Ddiab5a | AUC_10wks | 11.89 | 141.54 | 139.55 | 143.37 | 0 | 0 | sd | 46 | Gper |
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| 6 | Ddiab6a | AUC_20wks | 3.24 | 48.94 | 48.02 | 51.57 | 0 | sd | 0 | 44 | Igf2bp3 |
| Ddiab6b | bGLC_20wks | 2.86 | 78.00 | 77.21 | 79.74 | 0 | sd | sd | 7 | ||
| Ddiab6c | AUC_20wks | 7.32 | 88.54 | 84.28 | 91.81 | sd | sd | sd | 112 | Alms1, Gfpt1, Tpra1, Klf15 | |
| Ddiab6d | INS | 8.85 | 107.02 | 100.75 | 115.43 | sd | sd | 0 | 65 | Edem, Ghrl, Pparg | |
| nGLC | 3.19 | 107.02 | sd | 1 | 0 | ||||||
| AUC_2 0wks | 3.43 | 107.21 | sd | 0 | d | ||||||
|
| |||||||||||
| 7 | Ddiab7a | INS | 2.75 | 26.18 | 25.25 | 26.60 | 0 | sd | 0 | 53 | Gsk3a, Lipe |
| Ddiab7b | AUC_10wks | 3.01 | 52.12 | 48.49 | 58.32 | 1 | d | 0 | 222 | Nr1h2, Akt1s1, Trpm4, Gys1, Fgf21, Kcnj11, Abcc8 | |
| AUC_20wks | 3.65 | 56.01 | 0 | sd | 0 | ||||||
| Ddiab7c | bGLC_10wks | 2.87 | 69.70 | 65.96 | 73.76 | 1 | 0 | s | 39 | Atp10a | |
| INS | 2.76 | 72.38 | 1 | 0 | d | ||||||
| Ddiab7d | AUC_20wks | 4.90 | 79.00 | 78.13 | 80.14 | 0 | sd | 0 | 1 | ||
|
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| 8 | Ddiab8a | AUC_10wks | 4.26 | 29.36 | 21.55 | 39.08 | sd | 0 | sd | 132 | Ikbkb, Adrb3 |
| Ddiab8b | AUC_10wks | 4.11 | 71.23 | 64.98 | 75.50 | sd | 0 | sd | 148 | Cpe, Lpl, Npy1r, Pik3r2, Rab3a, Slc27a1 | |
|
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| 9 | Ddiab9a | AUC_10wks | 3.66 | 15.64 | 15.12 | 18.02 | 1 | 0 | sd | 8 | |
| Ddiab9b | AUC_20wks | 3.02 | 54.42 | 52.12 | 56.50 | 1 | 0 | sd | 45 | ||
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| 10 | Ddiab10a | AUC_20wks | 2.96 | 28.07 | 24.01 | 32.45 | s | 0 | 0 | 31 | Enpp1, Med23 |
| Ddiab10b | INS | 2.70 | 105.44 | 104.95 | 107.74 | d | sd | 0 | 13 | ||
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| 11 | Ddiab11a | INS | 4.98 | 7.32 | 6.46 | 7.85 | sd | 0 | sd | 10 | Igfbp1, Igfbp3 |
| Ddiab11b | INS | 8.15 | 12.52 | 11.51 | 13.77 | sd | 1 | sd | 5 | Grb10 | |
| Ddiab11c | AUC_20wks | 2.88 | 80.69 | 80.01 | 81.60 | 0 | sd | 0 | 12 | ||
| Ddiab11d | AUC_20wks | 3.15 | 105.13 | 104.43 | 106.39 | 0 | 0 | sd | 31 | Gh | |
| Ddiab11e | AUC_20wks | 3.03 | 108.38 | 107.61 | 109.26 | 0 | 0 | 1 | 10 | ||
|
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| 12 | Ddiab12a | nGLC | 2.98 | 52.49 | 49.63 | 55.02 | 0 | 0 | 1 | 16 | |
| Ddiab12b | nGLC | 2.93 | 55.70 | 55.12 | 57.08 | 0 | 1 | 1 | 23 | Insm2 | |
| Ddiab12c | AUC_20wks | 3.50 | 72.09 | 71.01 | 72.87 | sd | 0 | 0 | 14 | ||
| Ddiab12d | AUC_20wks | 2.68 | 77.40 | 76.99 | 79.31 | sd | d | 0 | 18 | ||
| Ddiab12e | AUC_20wks | 2.78 | 103.77 | 103.00 | 104.13 | sd | 0 | 0 | 16 | ||
| Ddiab12f | AUC_10wks | 2.82 | 121.12 | 118.32 | 123.92 | 0 | 0 | sd | 10 | Ptprn2 | |
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| 13 | Ddiab13a | AUC_20wks | 3.06 | 29.67 | 21.92 | 33.36 | sd | 0 | 0 | 152 | |
| Ddiab13b | bGLC_10wks | 2.70 | 40.51 | 39.41 | 41.83 | 0 | s | d | 12 | ||
| Ddiab13c | bGLC_10wks | 2.73 | 61.03 | 57.90 | 62.99 | d | d | d | 52 | ||
| Ddiab13d | INS | 4.56 | 68.92 | 67.81 | 73.33 | 0 | d | sd | 20 | ||
| Ddiab13e | AUC_20wks | 3.24 | 87.10 | 86.46 | 88.39 | sd | s | 0 | 0 | ||
| Ddiab13f | AUC_20wks | 3.83 | 96.15 | 93.17 | 97.71 | sd | s | 0 | 37 | ||
| Ddiab13g | bGLC_10wks | 3.07 | 105.42 | 104.62 | 107.09 | 0 | s | s | 15 | ||
| Ddiab13h | AUC_10wks | 4.75 | 114.70 | 112.87 | 119.12 | sd | 0 | sd | 30 | ||
| AUC_20wks | 2.91 | 116.61 | sd | s | sd | ||||||
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| 14 | Ddiab14a | AUC_10wks | 4.02 | 23.78 | 23.24 | 29.48 | sd | 0 | sd | 31 | |
| Ddiab14b | INS | 3.09 | 31.66 | 28.31 | 34.68 | sd | sd | sd | 66 | ||
|
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| 15 | Ddiab15a | AUC_10wks | 3.43 | 21.94 | 21.94 | 23.61 | 0 | sd | sd | 2 | |
| Ddiab15b | AUC_10wks | 3.63 | 31.47 | 30.83 | 32.63 | 0 | 0 | sd | 11 | ||
| Ddiab15c | INS | 3.47 | 34.26 | 31.47 | 35.03 | d | d | 0 | 20 | ||
| Ddiab15d | AUC_10wks | 5.30 | 66.84 | 66.00 | 68.85 | d | sd | 0 | 12 | ||
|
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| 16 | Ddiab16a | AUC_10wks | 2.85 | 5.28 | 3.99 | 8.33 | 0 | 1 | sd | 32 | |
| Ddiab16b | INS | 2.58 | 50.30 | 49.00 | 50.37 | 0 | 0 | sd | 5 | ||
| Ddiab16c | INS | 2.60 | 51.06 | 50.44 | 58.54 | sd | sd | sd | 29 | ||
| AUC_20wks | 3.40 | 51.26 | 50.44 | 58.54 | 0 | sd | 0 | ||||
| Ddiab16d | INS | 3.44 | 56.02 | 53.44 | 59.52 | sd | sd | sd | 63 | ||
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| 17 | Ddiab17a | AUC_20wks | 2.56 | 10.92 | 10.40 | 12.31 | sd | 0 | 0 | 3 | |
| Ddiab17b | AUC_10wks | 2.85 | 25.48 | 23.81 | 25.90 | 0 | 0 | sd | 104 | Pdpk1, Sstr5 | |
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| 18 | Ddiab18a | AUC_10wks | 5.15 | 33.96 | 31.33 | 35.20 | sd | sd | 0 | 43 | Egr1 |
| Ddiab18b | bGLC_20wks | 3.19 | 57.67 | 56.90 | 58.74 | s | 0 | 0 | 12 | ||
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| 19 | Ddiab19a | nGLC | 2.67 | 18.58 | 17.08 | 20.01 | 0 | sd | sd | 10 | |
*
AUC_10wks: area under the curve at 10 weeks; AUC_20wks: area under the curve at 20 weeks; bGLC_10wks: basal glucose level at 10 weeks; bGLC_20wks: basal glucose level at 20 weeks; GLC: serum glucose level at necropsy; INS: serum insulin level at necropsy. Bold LOD scores indicate QTL significant at the genome-wide threshold, others are significant at their respective chromosome-wise thresholds.
¶
ADD: additive genotypic effect; DOM: dominance genotypic effect; IMP: imprinting genotypic effect; s: sex interaction; d: diet interaction; sd: sex-by-diet interaction; 1: main effect with no interaction; 0: no effect.