Table 1.

PCR primers for HPV testing

Name	Sequence 5′ to 3′
GAPDH Forward	GCTCATTTGCAGGGGGGAGCC
GAPDH Reverse	CTGATGATCTTGAGGCTGTTG
GAPDH Probe	CY5-TCTGCCCCCTCTGCTGATGCCCCCATGTTCGTCATGGGA –BHQ2
HPV 16 Forward	TTGCAGATCATCAAGAACACGTAGA
HPV 16 Reverse	GTAGAGATCAGTTGTCTCTGGTTGC
HPV 16 Probe	JOE-AATCATGCATGGAGATACACCTACATTGCATGA –BHQ1
HPV 18 Forward	CAACCGAGCACGACAGGAACG
HPV 18 Reverse	TAGAAGGTCAACCGGAATTTTCAT
HPV 18 Probe	ROX-AATATTAAGTATGCATGGACCTAAGGCAACATTGCAA –BHQ2
HPV all Forward (E1)	CCTATAGTACATTTAAAAGGTG
HPV all Reverse (E1)	CNTGTCCAATGCCAGGTAGATG
HPV all Probe (E1)	FAM-AATAGTTTAAAATGTTTAAGATATAG-BHQ1
HPV other Forward (E2)	GCATTATATTGGTATAGAACAGG
HPV other Reverse (E2)	TCATTRTCASATGCCCATTGYACC

HPV human papillomavirus; R puRine (A or T); S Strong (C or G); Y pYrimidine (C or T).