Table 3. Antibody information.
Antibody | Company | Catalogue number | Dilution |
BLBP | Abcam | Ab32423 | 1∶500 |
CD133 | eBioscience | 14-1331-80 | 1∶50 (1∶500 sections) |
N-cadherin | Santa Cruz | SC7939 | 1∶200 |
Nestin 130 | [79] | 1∶1000 | |
Notch1-C20 | Santa Cruz | Sc-6014 | 1∶500 |
Pard3 | Millipore | 07–330 | 1∶500 |
Pax6 | Millipore | Ab2237 | 1∶500 |
PKCz | Abcam | Ab59412 | 1∶200 |
Secondary antibodies | Molecular Probes | 1∶200 | |
Sox2 | Santa Cruz | SC1730X | 1∶200 |
Sox2 | Millipore | Ab5603 | 1∶500 |
Sox3 | Kind gift from Prof Jonas Muhr | 1∶1000 | |
TuJ1 | Promega | G712A | 1∶1000 |
ZO1 | Invitrogen | 402200 | 1∶500 (1∶50 sections) |