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. 2013 May 10;8(5):e64231. doi: 10.1371/journal.pone.0064231

Figure 2. DDRGK1 regulates the expression of cyclin D1.

Figure 2

(A) The depletion of DDRGK1 inhibits the expression of cyclin D1 in MCF-7 cells. MCF-7 cells were transfected with mixed DDRGK1 siRNAs or a control siRNA. The expression of DDRGK1 and four cyclin genes were analyzed by RT-PCR. GAPDH was used as a loading control. (B) The depletion of DDRGK1 decreases the expression of cyclin D1. MCF-7 cells and U2OS cells were transfected with mixed DDRGK1 siRNAs or a control siRNA. The level of cyclin D1 protein was determined by immunoblotting. (C) The depletion of DDRGK1 decreases the cyclin D1 promoter activity. U2OS cells and MCF-7 cells were transfected with mixed DDRGK1 siRNAs or a control siRNA along with the cyclin D1 promoter reporters. Relative luciferase activity was determined by the dual luciferase assay system. In this analysis and subsequent luciferase assays, firefly luciferase activity was normalized with the Renilla luciferase reporter of pRL-TK. The data are expressed as the mean±standard error from three independent experiments.