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. 2013 May 10;8(5):e63138. doi: 10.1371/journal.pone.0063138

Figure 2. Alk1-null pulmonary ECs displayed elevated migratory index upon angiogenic factor challenge.

Figure 2

A. Time-lapse images of scratch-wound closure of 2f/1f- and 1f/1f-ECs in response to bFGF (50 ng/ml). At 12 hours post-wounding, mutant cells almost completely closed the wound, while the wound was still present in the control culture. B. Statistical analysis shows that the rate of wound closure in 1f/1f-ECs was higher than that in 2f/1f-ECs. C. Migration distance of 1f/1f-ECs at the 12-hour time point was higher than that of 2f/1f-ECs. D. In the 3D modified Boyden chamber assay, significantly more migrating 1f/1f-ECs were counted in six randomly chosen fields as compared with 2f/1f-ECs. Note that all data represent means from three independent experiments. Error bars show standard errors. *p<0.05.