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. 2013 May 10;8(5):e63138. doi: 10.1371/journal.pone.0063138

Figure 5. LDN-193189 treatment efficiently block BMP9 mediated SMAD1/5/8 phosphorylation in 1f/1f-ECs.

Figure 5

2f/2f- and 1f/1f-ECs were deprived of serum for 24 hours in chemically defined growth factor- and serum-free ECM, and cells were treated with solvent control (PBS, pH 7.4 with 2% 2-hydroxypropyl-b-cyclodextrin; A) or LDN-193189 (100 nM; B) 2 hours prior to treating cells with BMP9 (0, 0.1, 0.5, 1,5, or 20 ng/ml) for 30 minutes. β-Actin was used as a loading control. Histogram shows phospho-SMAD1/5/8 normalized by total SMAD1 and β-Actin relative to the value indicated by asterisks. Data in all graphs represent means of values measured by densitometry from three separate blots. Error bars are SDs (**p<0.01).