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. 2013 May 10;8(5):e64190. doi: 10.1371/journal.pone.0064190

Figure 1. Molecular analysis of PSARK::IPT-transgenic cotton.

Figure 1

A. PCR analysis of PSARK::IPT-transgenic cotton plants using the SARK promoter specific forward primer and the IPT specific reverse primer. WT, wild-type; 1, 2, 5, 6, 7, and 9, six independent PSARK::IPT-transgenic cotton plants. B. RNA blot analysis of wild-type and PSARK::IPT-transgenic cotton plants using an IPT DNA fragment as a probe. C. Relative IPT expression in two PSARK::IPT-transgenic cotton plants under well watered and water-deficit conditions. The quantitative RT-PCR experiments were conducted using the cotton ubiquitin gene UBQ7 as the internal standard. SNT, segregating non-transgenic. D. DNA blot analysis of wild-type, segregating non-transgenic, and four PSARK::IPT-transgenic cotton plants. M, DNA molecular size marker.