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. 2013 May 10;8(5):e63465. doi: 10.1371/journal.pone.0063465

Figure 5. Phosphorylation of p65.

Figure 5

A–C Confocal immunofluorescence images show phosphorylation of the NF-κB p65 subunit in lung microvessels (green) and fluorescence of the nuclear marker Hoechst-33342 (blue) for the indicated treatments. Treatment durations and agent concentrations were as outlined in Methods. Scale bar = 20 µm. D, E Bar graphs show NF-κB p65 phosphorylation levels along the vessel wall over the length of single microvessels for both venules (D) and capillaries (E). Mean±SE. n - number of vessels analyzed per group. Each treatment repeated in 3 lungs each. *-p<0.05 compared to HBS and XeC+LPS groups.