Table 1. Composition of control and protein-restricted (8%) diet.
| Control dieta | Protein-restricted dietb | |
| Ingredients (g/Kg) | ||
| Soybean+wheat | 230.0 | 80.0 |
| Corn starch | 676.0 | 826.0 |
| Soybean oil | 50.0 | 50.0 |
| Vitamin mixc | 4.0 | 4.0 |
| Mineral mixc | 40.0 | 40.0 |
| Macronutrient composition (%) | ||
| Protein | 23.0 | 8.0 |
| Carbohydrate | 66.0 | 81.0 |
| Fat | 11.0 | 11.0 |
| Total energy (Kj/Kg) | 17038.7 | 17038.7 |
a
Standard diet for rats (Nuvilab – NUVITAL Nutrientes LTDA, Paraná, Brazil).
b
The protein restricted diet was prepared in our laboratory using the control diet and replacing part of its protein with cornstarch. The amount of the latter was calculated so as to make up for the decrease in energy content due to protein restriction.
c
Vitamin and mineral mix were formulated according to the American Institute of Nutrition 93-G recommendation for rodents diets [31].