Table 1.

Antibodies used in immunohistochemistry for p16 and cyclin D1

Marker	Dilution	Incubation	Staining pattern	Positive control	Negative control	Catalogue number
p16	Ready to use	Room temperature, 30 min	Nuclear and cytoplasmic	SiHa	Muscle	CINtec® Histology Kit (Roche mtm laboratories AG, Heidelberg, Germany)
Cyclin D1	1:35	4°C, Overnight	Nuclear	SCC-9	Muscle	Cyclin D1 (92G2) Rabbit mAb #2978 (Cell Signaling, Ontario, CA)