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. 2013 May 10;8(5):e63152. doi: 10.1371/journal.pone.0063152

Figure 1. Cellular ROS levels are increased in bone marrow cells of Ptpn11 GOF mutant mice.

Figure 1

(A) Bone marrow cells freshly harvested from five-month-old Ptpn11D61G/+ and Ptpn11+/+ mice (n = 5/group) were loaded with 2′-7′-dichlorofluorescein diacetate (DCF-DA). In addition, bone marrow cells were treated with H2O2 (200 µM). Intracellular ROS levels were quantified by FACS. (B) Four-week-old Ptpn11E76K neo/+/Mx1-Cre+ and Ptpn11+/+/Mx1-Cre+ mice were treated by intraperitoneal injection of a total of 5 doses of polyinosine-polycyticyclic acid (pI-pC) (250 µg/mouse) administered every other day over 10 days as we previously described [29]. Twelve weeks after pI-pC treatment, bone marrow cells were harvested from Ptpn11E76K/+ and Ptpn11+/+ mice (n = 5/group). Intracellular ROS levels were quantified by FACS as above.