Figure 5. Mitochondrial aerobic metabolism is enhanced by Ptpn11 GOF mutation.

Four-week-old Ptpn11^{E76K neo/+}/Mx1-Cre+ and Ptpn11^+/+/Mx1-Cre+ mice were treated with pI-pC as described in Figure 1B. Twelve weeks after pI-pC treatment, bone marrow-derived macrophages were generated from bone marrow cells isolated from these mice (n = 3/group). Oxygen consumption rates (OCR) (A) and extracellular acidification rates (ECAR) (B) of intact macrophages were measured using the Seahorse XF24 analyzer in the presence of the mitochondrial inhibitor (oligomycin, 350 nM), the uncoupling agent (FCCP, 5 µM), and the respiratory chain inhibitor (rotenone, 1 µM).