TABLE I.
Sequences of oligonucleotides (DNA/aptamers) used in the present work.
| DNA/aptamers | Abbreviations | Sequences from 5′ to 3′ |
|---|---|---|
| Thrombin aptamer for capturing | TBA1 | GGTTGGTGTGGTTGG |
| Biotinylated TBA1 | b-TBA1 | Biotin-C6-GGTTGGTGTGGTTGG |
| FAM a linked biotinylated TBA1 | b-TBA1-FAM | Biotin-C6-GGTTGGTGTGGTTGG-FAM |
| Thrombin aptamer for signal enhancement | TBA2 | AGTCCGTCCTAGGGCAGGTTGGGGTGACT |
| TBA2 with 10 thymine bases | TBA2-10T | T10-AGTCCGTCCTAGGGCAGGTTGGGGTGACT |
| TBA2 with 20 thymine bases | TBA2-20T | T20-AGTCCGTCCTAGGGCAGGTTGGGGTGACT |
| Biotinylated ssDNA | b-ssDNA | Biotin-C6-TTCTTTCTTCCCCTTGTTTGTT |
a
FAM: (5-)6-carboxy-fluorescein.