Table 1.
Murine anti-human PSA IgG1 titers in the serum of vaccinated human FcεRIα transgenic mice
| Titers 14 days after initial immunization | ||||
|---|---|---|---|---|
| Mouse # |
PSA + anti-PSA IgE |
PSA + anti-PSA IgG1 |
PSA |
Buffer |
| 1 |
8,100 |
100 |
900 |
0 |
| 2 |
2,700 |
24,300 |
0 |
0 |
| 3 |
900 |
8,100 |
0 |
0 |
| 4 |
2,700 |
24,300 |
0 |
0 |
| 5 |
2,700 |
24,300 |
2,700 |
0 |
| 6 |
900 |
72,900 |
8,100 |
0 |
| 7 |
24,300 |
8,100 |
900 |
0 |
| 8 |
900 |
2,700 |
900 |
0 |
| Median |
2,700 |
16,200 |
900 |
0 |
|
Titers 13 days after booster (28 days after initial immunization) | ||||
| Mouse # |
PSA + anti-PSA IgE |
PSA + anti-PSA IgG1 |
PSA |
Buffer |
| 1 |
218,700 |
8,100 |
24,300 |
0 |
| 2 |
218,700 |
72,900 |
0 |
0 |
| 3 |
72,900 |
24,300 |
0 |
0 |
| 4 |
218,700 |
218,700 |
0 |
0 |
| 5 |
72,900 |
400,000 |
218,700 |
0 |
| 6 |
72,900 |
218,700 |
218,700 |
0 |
| 7 |
24,300 |
218,700 |
8,100 |
0 |
| 8 |
218,700 |
24,300 |
72,900 |
0 |
| Median | 145,800 | 145,800 | 16,200 | 0 |
Data shown are combined from two independent experiments (n = 4) with a total of 8 animals per group. Sera were serially diluted 3-fold starting at 1:100. Titer was determined as the highest dilution from which a positive signal was obtained.