Table 1.
Comparison of LAMP and real-time PCR for detection of orf from intracutaneous and cohabitation infections
The route of infection |
Samples |
Days after infection |
Assay |
|
---|---|---|---|---|
LAMP | real-time PCR | |||
Intracutaneous |
Goat 1 |
10 |
+ |
+ |
11 |
+ |
+ |
||
12 |
+ |
+ |
||
13 |
+ |
+ |
||
14 |
+ |
+ |
||
15 |
+ |
+ |
||
16 |
+ |
+ |
||
17 |
+ |
+ |
||
18 |
+ |
+ |
||
20 |
+ |
+ |
||
22 |
+ |
+ |
||
25 |
- |
- |
||
28 |
+ |
+ |
||
Goat 2 |
12 |
+ |
+ |
|
13 |
+ |
+ |
||
14 |
+ |
+ |
||
15 |
+ |
+ |
||
16 |
+ |
+ |
||
17 |
+ |
+ |
||
18 |
+ |
+ |
||
19 |
+ |
+ |
||
20 |
+ |
+ |
||
23 |
+ |
+ |
||
25 |
+ |
+ |
||
27 |
+ |
+ |
||
30 |
- |
+ |
||
Cohabitation |
Goat 3 |
18 |
+ |
+ |
21 |
+ |
+ |
||
22 |
+ |
+ |
||
23 |
+ |
+ |
||
24 |
+ |
+ |
||
25 |
+ |
+ |
||
26 |
+ |
+ |
||
27 |
- |
- |
||
29 |
+ |
+ |
||
Goat 4 |
17 |
+ |
+ |
|
19 |
+ |
+ |
||
20 |
+ |
+ |
||
21 |
+ |
+ |
||
22 |
+ |
+ |
||
23 |
+ |
+ |
||
24 |
+ |
+ |
||
25 |
+ |
+ |
||
27 |
+ |
+ |
||
29 |
- |
- |
||
32 | - | - |
+, Positive. -, Negative.